What is the MPNPLATE?

The Colitag MPNplate™ is a simple and easy to use device for enumerating microorganisms in a liquid sample. It is a modern approach to the traditional 15 tube serial dilution method and the statistical model of the most probable number (MPN) index, as published in the Standard Methods for the Examination of Water and Wastewater (American Public Health Association, American Water Works Association, and Water Environmental Federation) and accepted worldwide. Replacing the time consuming and labor intensive 15-tube dilution method, the device has five 10 mL wells, five 1 mL wells, and five 0.1 mL wells. A 16th well for collecting the remaining sample allows a full 100 mL sample to be analyzed. A mixture of Colitag reagent and sample is poured directly into the wells, filling all 15 wells first and the overflow well after. The device is then sealed with an adhesive film and set up for incubation. Result reading and MPN determination is direct and simple.

How does it work?

Tests using Colitag™ and the MPNplate™ offer a high degree of sensitivity with a detection limit ranging from <1 to 1,600 MPN per 100 mL sample. The Colitag™ MPN plate™ system requires only an incubator, a long-wave UV lamp, a reagent blank or control strains, sealing film, and the hand-held sealing tool. The entire procedure involves only a few steps and can be done in less than one minute per sample.

How can I order MPNplates?

For ordering and for information about validating reagents/test methods using the MPNplate™ or for technical support, please contact Neogen®.

Part NumberDescription
4600-PLATEFILM100MPN Plate, with Sealing Films, 100pk
4600-PLATEFILM20MPN Plate, with Sealing Films, 20pk

Get started using Colitag™. Click here to receive a PDF copy of the Colitag™ Certification Requirements. The MPNPlate is not currently EPA approved and should only be used for non-compliance enumeration testing.